PAST CILIA EVENTS 2020

17th BSCB GenSoc UK Cilia Network e-symposium

Date: 15 December 2020 Time: 15:00 - 17:30

Gonzalo Ortiz Alvarez, postdoc, Spassky & Meunier labs (Institut de Biologie de l'École Normale Supérieure, INSERM, FR) 'DNA replication regulators and multiciliogenesis in the mouse brain'. Alessia Di Nardo, postdoc, Sahin lab (Boston Children's Hospital, Harvard, US) 'A Phenotypic Screen in Tuberous Sclerosis Complex reveals druggable pathways for mTORC1 and primary cilia' . Andrew Streets, postdoc, Ong Lab (University of Sheffield, UK) 'Polycystin-1 regulates ARHGAP35-dependent centrosomal RhoA activation and ROCK signalling'. Travis Walton, postdoc, Brown Lab (Harvard University, US) 'Structure of Outer Dynein Arms Natively Bound to Doublet Microtubules'. Nina Schweizer, postdoc, Luders Lab (IRB Barcelona, ES) 'Sub-centrosomal mapping identifies augmin-gTuRC as part of a centriole-stabilizing scaffold'. Peter Jackson, PI (Stanford University, US) 'Ciliary Control Of Pancreatic Secretion'.

BSCB GenSoc UK Cilia Network e-Symposium 16

24/11/2020 15:00-17:15 BST

Olatz Pampliega, PI (Achucarro Basque Center for Neuroscience, ES) 'Amyloid beta modulates neuronal autophagy through the primary cilium'; Adam Soh, PhD student Pearson lab (University of Colorado Anschutz Medical Campus, US) 'Tetrahymena thermophila basal body connections promote ciliary synchrony'; Gabrielle Wheway, PI (University of Southampton, Faculty of Medicine CA) 'Discovery of a novel isoform of SARS-CoV-2 receptor Angiotensin Converting Enzyme-2 (ACE2)'; Hisham Bazzi, PI (University of Cologne, DE) 'Gradual centriole maturation is associated with the mitotic surveillance pathway establishment during mouse development'; Maria Teves, PI (Virginia Commonwealth University, US 'Reduced expression of SPAG17 links dysfunctional primary cilia, morphogen signaling activation and multiple organ fibrosis'; Andrew Holland, PI (Johns Hopkins University School of Medicine, US) 'Centriole distal appendages act as signaling platforms that control cell proliferation'.

BSCB GenSoc UK Cilia Network e-Symposium 15

10/11/2020 15:00-17:15 BST

Wang Kyaw Twan, (recent PhD student Hamada lab, RIKEN BDR, JP) 'Cfap53 regulates mammalian cilia-type motility patterns through differential localization and recruitment of axonemal dynein components'. Nikolai Klena, (PhD student Guichard/Hamel lab, University of Geneva, CH) 'Probing the evolutionary conservation of the centriole’s cartwheel-containing region by cryo-electron tomography'. Jantje Gerdes, (PI, Helmholtz Munich, DE) 'Primary cilia and beta-cell function'. Laura Devlin, (PhD student, Sayer lab, University of Newcastle, UK) 'Inactivation of Cep164 in adult mice reveals complex, mixed ciliopathy phenotypes'. Tara Akhshi, (recent PhD student, Trimble lab, University of Toronto, CA) 'Non-Canonical Hedgehog Activity Initiates Ciliogenesis via LKB1-AMPK and Gi-LGN-NuMA-Dynein Axes'.

BSCB GenSoc UK Cilia Network e-Symposium 14

27/10/2020 15:00-17:15 BST

Hugo van den Hoek, PhD student Engel lab (Helmholtz Centre Munich, DE) 'Transition zone in situ: cryo-electron tomography reveals IFT train assembly at the ciliary base'. Marine Laporte, post-doc Guichard/Hamel lab (University of Geneva, CH) 'The evolutionary conserved role of the SFI1/centrins modules revealed in human centriole'. Ilaria Dutto, postdoc, Stracker lab (IRB Barcelona, ES) 'ADSL deficiency phenotypes in vitro and in vivo'. Justine Pinksey, postdoc, Nicastro lab (UT Southwestern Medical Center, US) 'Choanoflagellate Cilia Structures Revealed by Cryo-Electron Tomography'. Paurav Desai, postdoc, Pazour lab (University of Massachusetts Medical, US) 'Ubiquitin regulates ciliary Smoothened levels'. Eszter Vladar, PI (University of Colorado Anschutz Medical Campus, US) 'Ciliated cell dysfunction and COVID-19 lung disease'.

BSCB GenSoc UK Cilia Network e-Symposium 13

13/10/2020 15:00-17:15 BST

Awesome session of all things cilia: diversity of ependymal motile cilia; primary cilia in meiosis; mysterious distal centrioles; resolving structures required for motility; cytoskeletal regulators and Hh signaling PLUS how signaling regulates sensory cilia size and shape!

Farah Haque, postdoc Subramanian lab (Harvard University, US) 'Gli(ee)ful mimicry'. Yaniv Elkouby, PI (The Hebrew University of Jerusalem Faculty of Medicine, IL) 'A novel primary cilium mechanically regulates meiotic chromosomal pairing and reinforces germline cyst morphogenesis' . Miao Gui, postdoc, Brown lab (Harvard University, US) 'Structural insights into the mechanoregulatory network that controls ciliary motility'. Dhivya Kumar, postdoc, Reiter lab (UCSF, US) 'The distal centriole: the nexus between the cilium and centriole'. Ashish Maurya, postdoc, Sengupta lab (Brandeis University, US) 'The activity of a conserved kinase cascade determines sensory cilia size and elaboration'. Nathalie Jurisch-Yaksi, PI (Norwegian University of Science and Technology, NO) 'Molecular and cellular properties of ependymal cells in the zebrafish brain'.

BSCB GenSoc UK Cilia Network e-Symposium 12

29/09/2020 15:00-17:15 BST

Another phenomenal line-up: translational control in ciliogenesis; SMO and atypical signaling; Rab19, actin and ciliogenesis; autoimmune SSNA and microtubule dynamics; resolving the central pair complex and exciting ECVs from cilia tip and base!

We kick off our world tour with Jacinta D’Souza, PI (UM-DAE Centre for Excellence in Basic Sciences, IN) ‘Identification of an Adenylate kinase-rich AKAP anchored central pair-based multiprotein complex from the Chlamydomonas flagella’, Ben Myers, PI (University of Utah School of Medicine, US) ’Smoothened Transduces Hedgehog Signals via Activity-Dependent Sequestration of PKA Catalytic Subunits’ , Cayla Jewett, PhD student, Prekeris lab (University of Colorado Anschutz Medical Campus, US) ‘Rab19 directs cortical remodeling and membrane deposition for primary ciliogenesis’, Patricia Yeyati, senior scientist (MRC Human Genetics Unit, UK) ‘Unravelling roles for translational control in motile ciliogenesis’, Beth Lawrence, postdoc, Zanic lab (Vanderbilt, US) ‘Sjögren's Syndrome Protein SSNA1 Suppresses Microtubule Dynamicity in vitro’ and last but not least Maureen Barr, PI (Rutgers, US) ‘The Fundamental Biology of Ciliary Extracellular Vesicles: Biogenesis, Cargo, Function, Targets’.

BSCB GenSoc UK Cilia Network e-Symposium 11

15/09/2020 15:00-17:15 BST

We had a fantastic line up for you: interrogating differences between cilia types in the brain at nanoscale resolution; mysterious roles for meiotic proteins in cilia function; modeling ciliopathy genes for mechanism; fine tuning cells for sensing Hh; plus cilia and signaling in Down’s Syndrome and in neural tube development!

We have the amazing Carolyn Ott, senior scientist, Lippincott-Schwartz lab (HHMI Janelia, US) ‘Volumetric electron microscopy reveals differences between neuronal and glia primary cilia’; Urszula McClurg, PI (Liverpool, UK) ‘New lessons from an old protein – how TEX12 controls centrosome and cilia and why would that make us skinny?‘, Chad Pearson, PI (CU Anschutz, US) ‘Microtubule and primary ciliary dysfunction in response to elevated chromosome 21 dosage, Jennifer Kong, post doc, Rohatgi lab (Stanford, US) ‘Molecular mechanisms that regulate target cell sensitivity to Hedgehog morphogens’, André Gonçalves, postdoc, Pedersen lab (Copenhagen, DK) ‘Molecular basis for ciliopathies caused by CEP78 mutations’ and Saikat Mukhopadhyay, PI (UT Southwestern Medical Centre, US) ‘Adenylyl cyclase signaling, cilia and neural tube development’

BSCB GenSoc UK Cilia Network e-Symposium 10

01/09/2020 15:00-17:15 BST

We literally blew it up with this exciting line-up: time-resolved cilia proteomes, revealing structure with expansion microscopy, centriolar satellites and proteostasis, cilia and centriole extrusion, dissecting axonemal microtubules and role of cilia in PKD! The awesome: Brian Mitchell, PI (Northwestern University, US) ‘Cilia and centriole extrusion in Xenopus mulitciliated cells', Virginie Hamel, co-PI, Centriole lab (University of Geneva, CH) ‘Revealing the architecture of centrioles using expansion microscopy and cryo-tomography’, Ron Orbach, postdoc, Howard lab (Yale University, US) ‘Dissecting the Axoneme Structure - Step by Step’, Suzy Prosser, postdoc, Pelletier lab (University of Toronto, CA) ‘An emerging role for centrosomal proteins in the regulation of proteostasis’, Rachel Gallagher, senior scientist, Somlo lab (Yale University, US) ‘Loss of cilia has no impact on disease progression in ARPKD' and David Mick, PI (Saarland University, DE). 'Time-resolved primary cilia proteomics to reveal the Hedgehog-induced remodeling of the cilium proteome’.

BSCB GenSoc UK Cilia Network e-Symposium 9

18/08/2020 15:00-17:15 BST

Another exciting line-up: redistibuting centriolar satellites, differential expression of motors, Parkinsons’ genes and vesicular trafficking, cilia-generated flow and curly spines, eye organoids and cilia and obesity! We have the phenomenal Elif Nur Firat-Karalar (Koç University, TR) ‘Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium', Petra zur Lage (Jarman lab, University of Edinburgh, UK) ‘Differences in localisation of axonemal dyneins within Drosophila cilia’, Suzanne Pfeffer (Stanford University US) ‘Regulation of ciliogenesis by LRRK2 kinase’, Anne Meyer Miner (Ciruna lab, University of Toronto, CA) ‘Investigating the role for motile ciliated cell lineages in spine morphogenesis’, Katarina Jovanovic (Cheetham lab, University College London, UK) ‘Modelling and rescue of RP2 Retinitis Pigmentosa in human retinal tissues' and ending on a bang Nick Berbari (Indiana University-Purdue University Indianapolis, US) 'Dysregulation of hedgehog signaling in adult neurons results in obesity'.

BSCB GenSoc UK Cilia Network e-Symposium 8

04/08/2020 15:00-17:15 BST

Our speakers are Sudipto Roy (Institute of Molecular and Cell Biology, SG) ‘Cilia, Reissner Fibre and Crooked Spines', Manon Rivagorda (Oury lab, Institut Necker Enfants Malades - INSERM, FR) 'Role of the neuronal primary cilia-autophagy axis in the regulation of cognition and brain plasticity', Karl Lechtreck, (University of Georgia, US) 'The Chlamydomonas PKD2 complex', Mohamed Antar Aziz Mohamed, (Ökten lab, Technische Universität München, DE) ‘OSM-3 motor recruitment and activation for intraflagellar transport', Yulu Cherry Liu (Hood College, US) ‘Glypican 3–derived heparan sulfate tunes Hedgehog signal strength post signal transduction’ and last but not least the amazing Radhika Subramanian, (Harvard University, US) ‘Visualizing the work of microtubule architects by Atomic Force Microscopy’.

BSCB GenSoc UK Cilia Network e-Symposium 7

21/07/2020 15:00-17:15 BST

This week: another exciting line up! Advanced imaging of cilia dynamics, novel regulators of cilia structure and function, mind-blowing ultrastructure, localized RNA at cilia, cilia signaling in axonal development and more! Our speakers are the amazing Jan Hansen (University of Bonn), Sarah Goetz (Duke), Khanh Huy Bui (McGill), Takashi Ochi (University of Leeds), Jaclyn Fingerhut (University of Michigan at Ann Arbor) and Jiami Guo (University of Calgary).

BSCB GenSoc UK Cilia Network e-Symposium 4

09/06/2020 15:00-17:15 BST This week: cilia and psychoactive substances, trafficking, advanced imaging, infertility, post-translational modifications & how a cell counts how many cilia are needed! Our cast of phenomenal speakers includes Jeremy McIntyre (University of Florida), Sylwia Niedziółka (University of Warsaw), Lucien Weiss (Technion Israel Institute of Technology), Clémence Belleannée (Université Laval), Sudarshan Gadadhar (Institut Curie) and Moe Mahjoub (WUSTL).

BSCB GenSoc UK Cilia Network e-Symposium 3

26/05/2020 15:00-17:15 BST This week: cilia and diabetes, DNA damage, neural development, motors & cilia loss! Our speakers are the amazing Jing Hughes (WUSTL), Ciaran Morrison (NUI Galway), Ashley Giam (University of Dundee), Kerstin Hasenpusch-Theil (University of Edinburgh) and Peter Walentek (University of Freiburg)

BSCB GenSoc UK Cilia Network e-Symposium 2

12/05/2020 15:00-17:15 BST This week: cilia and signaling, centriolar satellites, brain organoids, microtubules & cilia loss!

3:00 – 3:05 Pleasantine Mill Welcome Remarks

3:05 – 3:25 Lewis Mosby (Straube/Polin Labs, University of Warwick, UK) ‘Modelling EB-mediated cargo transport’

3:25- 3:45 Emma Hall (Mill Lab, MRC Human Genetics Unit, UK) ‘Centriolar satellite loss causes dwarfism and ciliopathy phenotypes.’

3:45 – 4:05 Thomas Theil (Centre for Discovery Brain Sciences, University of Edinburgh, UK) ‘The Joubert gene INPP5E acts as a negative regulator of Sonic Hedgehog signalling in human brain organoids’

4:05 - 4:25 Laura Dyer (Norris Lab, MRC Harwell, UK) ‘Unravelling the role of Polycystins in L-R pattern determination’

4:25 – 4:45 Jeremy Reiter (UCSF, USA) ‘How cells discriminate ciliary and nonciliary GPCR signaling’